Even though we have not tested PneumaCult™ media for use with murine epithelial cells, you will discover publications that report this software.

Combine gently before transferring cells to a conical tube made up of ten mL of pre-warmed wash medium. Note: Clean the pipette suggestion and cryovial Together with the wash medium not less than three instances to assist make improvements to recovery.

Movement cytometry can be employed to measure the performance of differentiation protocols While using the STEMdiff™ system, by checking out particular marker expression at Just about every stage of your differentiation method. It will also be utilized for other purposes, such as cell sorting, immunophenotyping, and purity evaluation.

Work quickly at this action to pool wells right into a 15 mL tube. Be aware: For a sizable-scale dissociation, use a multichannel pipette to pool cells right into a sterile reagent reservoir. Insert the pooled wells right into a 15 mL tube.

The STEMdiff™ system delivers a standardized course of action for differentiating hPSCs into epithelial cells that could be afterwards cultured in 2nd or 3D formats dependant upon the investigate needs.

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Evaluate the results of take a look at compounds on hematopoietic stem cells with phenotypic and purposeful assessments.

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Usually, HSAECs cultured in PneumaCult™-ALI-S Medium will variety a totally differentiated cuboidal epithelium right after four to 5 weeks of society. Some donor variability can be anticipated.

Use cell counts to different the sample into two tubes – “unstained” and “with viability dye”.

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The following fluorochrome-conjugated antibodies and dyes are recommended to facilitate 지방이식 Assessment of gene-edited CD34+ HSPCs: